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=”fig”}, **f**) and mean absolute error (MAE) values, as indicated in [Supplementary Table S1](#sup1){ref-type=”supplementary-material”}. Error bars represent mean and s.d.

BioNano Irys system
——————-

Mature microparticles (∼0.5 μm) were immobilized on a glass coverslip, and subsequently stained with fixative solution (0.5 [m]{.smallcaps} sucrose, 0.1 [m]{.smallcaps} imidazole, and 0.05% Triton X-100) for 30 min at room temperature. Fixed microparticles were washed with wash buffer (0.5 [m]{.smallcaps} sucrose, 0.1 [m]{.smallcaps} imidazole and 0.005% Triton X-100) three times before being stored at 4 °C.

Mapping of microparticle centres and diffusion rates
—————————————————–

Imaging of microparticles was performed on a BioNano Irys system (Iris II IN cell, Biomedical Platform, BioNano Genomics) for 15 min. Data from the DNA channel (488 nm) were collected every 20 s. Meta-data were saved using the integrated software, including coordinates of each microparticle, the number of imaging events, the diameter, as well as the location of the microparticle boundaries. Based on the above meta-data, the centre of mass and the average diffusion rate was calculated and exported as Excel files by the integrated software. All data were stored in bam and pst files and integrated into a single dataset using the Integrative Genomics Viewer[@dyu038-B43] (IGV) software. For calculating the exact centre of each microparticle, we first imported the raw Irys data in the IGV software, then the coordinates of the centre of each microparticle were exported as bam files using the ‘Export image’ command in the IGV software. The coordinates were imported into the SeqMonk suite (Version 0.36.2) software[@dyu038-B44] to calculate the centre of each microparticle.

Microscopy
———-

Field emission

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has to be in the right place at the right time. Every major league manager has had a series where his team took over a game and then just failed to hold on.

The Rays need to find a way to manufacture runs. It’s not surprising that they are one of the worst offensive teams in baseball. Their lineup is too top heavy. It’s something that you’ve got to be conscious of. No one is going to throw 3 solid hitters together every day. You have to be aware of that. Sometimes it’s just going to be a maddening series against a good pitching staff. It’s so hard to set that lineup up so you know that every player is going to get their fair share of plate appearances.

Unlike a lot of baseball, you can’t even predict the end of the season. It’s a very hard thing to do. If you could predict it, there’s an easy solution. But you can’t. Nobody knows. Just like you, I don’t know what’s going to happen in August. If you were a baseball fan in 1918, you wouldn’t know that you’d be in a World Series by now.

You never know what’s going to happen. So you’ve got to try to make the most of the opportunities that you’re given. You’re going to have your ups and downs. You’re going to have your peaks and your valleys. The thing is, at this point, you have to stick to your plan.

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